Journal: Cells

Article Title: NAD Modulates DNA Methylation and Cell Differentiation

doi: 10.3390/cells10112986

Figure Lengend Snippet: NAD induces myeloid differentiation in K562. ( a ) Wright Giemsa staining showing morphological changes between NAD-treated and control cells after four days. The figure is representative of five independent experiments ( b ) Representative image showing the increase in the surface markers CD15, CD14 and CD11b upon NAD treatment by flow cytometry. The table reports the mean % ±SD of CD14 + , CD15 + , CD11b + cells of five independent experiments. *: p < 0.05; **: p < 0.01; ***: p < 0.001 vs. vehicle ( n = 5) ( c ) NBT positive staining detected by small blue dots after counterstaining the cells with safranin. A magnification is shown in the rectangle. The pictures are representative of five independent experiments ( d ) Seahorse XF analysis of K562 mitochondrial stress response in cells treated with NAD or vehicle. The figure represents the mean of three biological replicates ( n = 3). Error bars indicate ± s.d.

Article Snippet: The cells were then stained with the Wright Giemsa solutions kit (CAMCO STAIN PAK Fort Lauderdale, FL, USA, pc#702) according to manufacturer’s instructions.

Techniques: Staining, Control, Flow Cytometry

Journal: Scientific Reports

Article Title: Si-based agent alleviated small bowel ischemia–reperfusion injury through antioxidant effects

doi: 10.1038/s41598-024-54542-7

Figure Lengend Snippet: Examination of goblet cells by mucin staining. ( a – d ) The representative microphotographs of the periodic acid-Schiff (PAS)-stained ( a , b ) or the Alcian blue-stained ( c , d ) or small intestine in the control (Con) ( a , c ) and Si groups ( b , d ). Scale bar: 40 µm ( a – d ). ( e , f ) The measured results of number of goblet cells in the damaged area in the Alcian blue-stained ( e ) or the PAS-stained ( f ) small intestine. Bar chart indicates the mean values of Goblet cells per villi. White: Con group. Grey: Si group. Data are expressed as mean. Standard error of the mean (SEM) of three (Con) or five mice (Si) per group. * p < 0.05 versus Con group, determined by Student’s paired t -test.

Article Snippet: Samples were stained using a PAS staining solution kit (ScyTek Laboratories, Inc., Logan, UT, USA) according to the manufacturer’s instructions.

Techniques: Staining, Control

Journal: bioRxiv

Article Title: NAD modulates DNA methylation and cell differentiation

doi: 10.1101/2020.07.31.231555

Figure Lengend Snippet: ( A ) Wright Giemsa staining showing morphological changes between NAD-treated and control cells after four days. ( B ) Increase in the surface markers CD15, CD14 and CD11b upon NAD treatment ( C ) NBT positive staining detected by small blue dots after counterstaining the cells with safranin. A magnification is shown in the rectangle. ( D ) Seahorse XF analysis of K562 mitochondrial stress response in cells treated with NAD or vehicle. The figure represents the mean of two biological replicates (n=2). Error bars indicate ± S.D. ( E ) Model showing the molecular mechanism of CEBPA gene reactivation by NAD. CEBPA is epigenetically silenced in K562. DNMT1 ensure a constant methylated status of CEBPA promoter (upper part). The NAD supplementation to K562 cell culture, boosts PARP1 to produce ADP-ribose polymers leading to DNMT1 inhibition (bottom part). The ultimate effect is CEPBA re-activated transcription.

Article Snippet: The cells were then stained with the Wright Giemsa solutions kit (CAMCO STAIN PAK, pc#702) according to manufacturer’s instructions.

Techniques: Staining, Methylation, Cell Culture, Inhibition